B Cell and Antibody Response to Seasonal Influenza Vaccines in Younger and Older Adults

Eligibility Criteria

• * Participation in ancillary clinical research study
• * Able to give informed consent
• * Age 18-50 years old for Arm 1 and Arm 2
• * Age 65-80 years old for Arm 3, Arm 4, and Arm 5
• * Weight of at least 110 lbs as determined by self-reporting
• * Inability to give informed consent
• * Refusal or inability to have blood drawn or participate in study procedures
• * Previous adverse reaction to influenza vaccine or medical history contraindicated for receiving influenza vaccine, including but not limited to:
• 1. History of Guillain-Barre Syndrome
• 2. History of egg allergy
• 3. History of gelatin allergy
• 4. History of moderate to severe illness with or without fever within 6 weeks of receipt of influenza vaccine
• * Previous receipt of influenza vaccine outside of study within current season
• * Bleeding disorder diagnosed by a doctor (eg, factor deficiency, coagulopathy, or platelet disorder requiring special precautions)
• * Participant has any medical, psychiatric, or social condition, or occupational or other responsibility that, in the judgement of the investigator would interfere with, or serve as a contraindication to the planned procedure(s).
• * These following criteria are used for scientific reasons, and not safety reasons. Specifically, the criteria are used to obtain a population that is healthy and less likely to have conditions that may influence the immune system:
• 1. No recent respiratory infections in the past 4 weeks at time of vaccination
• 2. Malignancy
• 3. Evidence of Inflammation: Systemic Lupus Erythematosis, Rheumatoid Arthritis, Polymyositis, Dermatomyositis, Scleroderma, Crohn's Disease, Ulcerative Colitis.
• 4. Lymphoproliferative Disorder
• 5. Known Immunodeficiency
• 6. Myocardial Infarction \<6 months
• 7. Cerebral Vascular Accident
• 8. Peripheral Vascular Disease- recannulation \<6months
• 9. Cardiac Insufficiency - congestive heart failure
• 10. Hypertension with increased blood urea nitrogen (BUN)
• 11. Renal Failure
• 12. Dementia
• 13. Alcoholism (defined as \>17 drinks/week)
• 14. Drug Abuse (excluding marijuana)
• 15. HIV positive
• 16. History of hepatitis
• 17. History of immunization within 4 weeks of study participation or plan to receive non- IIV vaccination within 4 weeks of receiving IIV
• 18. Moderate to severe illness at time of enrollment
• * Donations of blood in the 8 weeks prior to enrollment which, combined with expected volumes to be drawn for this study, would exceed 450 mL in an 8 week period.
• * Current pregnancy at time of enrollment or pregnancy within last 4 months
• * Active or planned breastfeeding during study participation
• * Psychiatric condition that precludes compliance with the protocol. Specifically excluded are persons with psychoses within the past 3 years, ongoing risk for suicide, or history of suicide attempt or gesture within the past 3 years.