RECRUITING

Autologous Mobilized Peripheral Blood CD34+ Hematopoietic Stem and Progenitor Cells (HSPC) Transduced With the Elongation Factor Alpha Short Promoter (EFS) - Adenosine Deaminase (ADA) Gene (EFS-ADA) Lentiviral Vector for Adenosine Deaminase Severe Combined Immune Deficiency (ADA SCID)

Conditions

Adenosine Deaminase Severe Combined Immune Deficiency Gene Therapy Hematopoietic Stem Cell Lentiviral Vector Reduced Intensity Conditioning with Busulfan

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Study Overview

This clinical trial focuses on testing the efficacy of different digital interventions to promote re-engagement in cancer-related long-term follow-up care for adolescent and young adult (AYA) survivors of childhood cancer.

Description

The aim of this study is to assess the safety and efficacy of autologous transplantation of hematopoietic stem cells (CD34+ cells) from mobilized peripheral blood (mPB) of ADA-deficient SCID infants and children following human ADA gene transfer by the EFS-ADA lentiviral vector. The level of gene transfer in blood cells and immune function will be measured as endpoints.

Official Title

Efficacy and Safety of Cryopreserved Autologous Mobilized Peripheral Blood CD34+ Hematopoietic Stem and Progenitor Cells Transduced Ex Vivo With the EFS-ADA Lentiviral Vector in Patients With Severe Combined Immune Deficiency Due To Adenosine Deaminase Deficiency

Quick Facts

Study Start:2023-01-04

Study Completion:2026-12-31

Study Type:Not specified

Phase:Not Applicable

Enrollment:Not specified

Status:RECRUITING

Study ID

NCT05432310

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Ages Eligible for Study:1 Month

Sexes Eligible for Study:ALL

Accepts Healthy Volunteers:No

Standard Ages:CHILD, ADULT, OLDER_ADULT

Inclusion Criteria	Exclusion Criteria
1. Provision of written informed consent prior to any study related procedures. In this study consent must be provided by the parents/legal guardians and, where applicable according to local laws, a signed assent from the child, 2. Subjects ≥30 days of age, 3. With a diagnosis of ADA-SCID based on: 1. Lymphopenia (absolute lymphocyte count (ALC) \<400 cells/mL) OR absence or low number of T cells (absolute CD3+ count \< 300 cells/mL), or 2. Severely decreased T lymphocyte blastogenic responses to phytohemagglutinin (either \<10% of lower limit of normal controls for the diagnostic laboratory, or \<10% of the response of the normal control of the day, or stimulation index \<10), or 3. Identification of SCID by neonatal screening revealing low T Cell Receptor Excision Circles (TREC) levels. 4. Ineligible for matched family allogeneic bone marrow (BM) transplantation, defined as the absence of a medically eligible HLA-identical sibling or family donor, with normal immune function, who could serve as an allogeneic bone marrow donor. 5. Females of child-bearing age will be required to provide a negative pregnancy test 30 days prior to Visit 2. 6. Subjects and their parents/legal guardians must be willing and able to comply with study restrictions and to remain at the clinic for the required duration during the study period and willing to return to the clinic for the follow up evaluation as specified in the protocol.	1. Ineligible for autologous HSCT as per clinical site criteria 2. Other conditions which in the opinion of the Principal Investigator and/or Co Investigators, contraindicate the mobilization of peripheral blood or the leukapheresis process, the administration of busulfan and the infusion of transduced cells, or which indicate an inability of the subject or subject's parent/legal guardian to comply with the protocol 3. Hematologic abnormality, defined as: * Anemia (Hb \<8.0 g/dl). * Neutropenia (ANC \<500/mm3). Note: ANC \<500 with absence of myelodysplastic syndrome on bone marrow aspirate and biopsy and normal marrow cytogenetics are acceptable for eligibility. * Thrombocytopenia (platelet count \<50,000/mm3, at any age). * Prothrombin time or international normalized ratio (INR) and partial thromboplastin time (PTT) \>2 x upper limit of normal (ULN) (subjects with a correctable deficiency controlled on medication will not be excluded). * Cytogenetic abnormalities on peripheral blood or bone marrow or amniotic fluid (if available). * Prior allogeneic HSCT with cytoreductive conditioning. 4. Pulmonary abnormality, defined as: * Resting O2 saturation by pulse oximetry \<90% on room air. * Chest X-ray indicating active or progressive pulmonary disease. Note: Chest X ray indicating residual signs of treated pneumonitis is acceptable for eligibility. 5. Cardiac abnormality, defined as: * Abnormal ECG indicating cardiac pathology. * Uncorrected congenital cardiac malformation with clinical symptoms. * Active cardiac disease, including clinical evidence of congestive heart failure, cyanosis, hypotension. * Poor cardiac function as evidenced by left ventricular ejection fraction \<40% on echocardiogram. 6. Neurologic abnormality, defined as: * Significant neurologic abnormality revealed by examination. * Uncontrolled seizure disorder. 7. Renal abnormality, defined as: * Renal insufficiency: serum creatinine ≥1.2 mg/dl (106 µmol/L), or ≥3+ proteinuria. * Abnormal serum sodium, potassium, calcium, magnesium or phosphate levels at \>2 x ULN. 8. Hepatic/gastrointestinal abnormality, defined as: * Serum transaminases \>5 x ULN. * Serum bilirubin \>2 x ULN. * Serum glucose \>1.5 x ULN. 9. Oncologic disease, defined as: * Evidence of active malignant disease other than dermatofibrosarcoma protuberans (DFSP). * Evidence of DFSP expected to require anti-neoplastic therapy within the 5 years following the infusion of genetically corrected cells (if anti-neoplastic therapy has been completed, a subject with a history of DFSP can be included). * Evidence of DFSP expected to be life limiting within the 5 years following the infusion of genetically corrected cells. 10. Known sensitivity to Busulfan. 11. Confirmation of an infectious disease by deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) positive at time of assessment for the following: * HIV-1, * Hepatitis B, * Parvovirus B19. 12. The subject is pregnant or has a major congenital anomaly. 13. Is likely to require treatment during the study with drugs that are not permitted by the study protocol. 14. The subject has previously received another form of gene therapy.

Inclusion Criteria

Exclusion Criteria

1. Provision of written informed consent prior to any study related procedures. In this study consent must be provided by the parents/legal guardians and, where applicable according to local laws, a signed assent from the child,
2. Subjects ≥30 days of age,
3. With a diagnosis of ADA-SCID based on:
1. Lymphopenia (absolute lymphocyte count (ALC) \<400 cells/mL) OR absence or low number of T cells (absolute CD3+ count \< 300 cells/mL), or
2. Severely decreased T lymphocyte blastogenic responses to phytohemagglutinin (either \<10% of lower limit of normal controls for the diagnostic laboratory, or \<10% of the response of the normal control of the day, or stimulation index \<10), or
3. Identification of SCID by neonatal screening revealing low T Cell Receptor Excision Circles (TREC) levels.
4. Ineligible for matched family allogeneic bone marrow (BM) transplantation, defined as the absence of a medically eligible HLA-identical sibling or family donor, with normal immune function, who could serve as an allogeneic bone marrow donor.
5. Females of child-bearing age will be required to provide a negative pregnancy test 30 days prior to Visit 2.
6. Subjects and their parents/legal guardians must be willing and able to comply with study restrictions and to remain at the clinic for the required duration during the study period and willing to return to the clinic for the follow up evaluation as specified in the protocol.

1. Ineligible for autologous HSCT as per clinical site criteria
2. Other conditions which in the opinion of the Principal Investigator and/or Co Investigators, contraindicate the mobilization of peripheral blood or the leukapheresis process, the administration of busulfan and the infusion of transduced cells, or which indicate an inability of the subject or subject's parent/legal guardian to comply with the protocol
3. Hematologic abnormality, defined as:
* Anemia (Hb \<8.0 g/dl).
* Neutropenia (ANC \<500/mm3). Note: ANC \<500 with absence of myelodysplastic syndrome on bone marrow aspirate and biopsy and normal marrow cytogenetics are acceptable for eligibility.
* Thrombocytopenia (platelet count \<50,000/mm3, at any age).
* Prothrombin time or international normalized ratio (INR) and partial thromboplastin time (PTT) \>2 x upper limit of normal (ULN) (subjects with a correctable deficiency controlled on medication will not be excluded).
* Cytogenetic abnormalities on peripheral blood or bone marrow or amniotic fluid (if available).
* Prior allogeneic HSCT with cytoreductive conditioning.
4. Pulmonary abnormality, defined as:
* Resting O2 saturation by pulse oximetry \<90% on room air.
* Chest X-ray indicating active or progressive pulmonary disease. Note: Chest X ray indicating residual signs of treated pneumonitis is acceptable for eligibility.
5. Cardiac abnormality, defined as:
* Abnormal ECG indicating cardiac pathology.
* Uncorrected congenital cardiac malformation with clinical symptoms.
* Active cardiac disease, including clinical evidence of congestive heart failure, cyanosis, hypotension.
* Poor cardiac function as evidenced by left ventricular ejection fraction \<40% on echocardiogram.
6. Neurologic abnormality, defined as:
* Significant neurologic abnormality revealed by examination.
* Uncontrolled seizure disorder.
7. Renal abnormality, defined as:
* Renal insufficiency: serum creatinine ≥1.2 mg/dl (106 µmol/L), or ≥3+ proteinuria.
* Abnormal serum sodium, potassium, calcium, magnesium or phosphate levels at \>2 x ULN.
8. Hepatic/gastrointestinal abnormality, defined as:
* Serum transaminases \>5 x ULN.
* Serum bilirubin \>2 x ULN.
* Serum glucose \>1.5 x ULN.
9. Oncologic disease, defined as:
* Evidence of active malignant disease other than dermatofibrosarcoma protuberans (DFSP).
* Evidence of DFSP expected to require anti-neoplastic therapy within the 5 years following the infusion of genetically corrected cells (if anti-neoplastic therapy has been completed, a subject with a history of DFSP can be included).
* Evidence of DFSP expected to be life limiting within the 5 years following the infusion of genetically corrected cells.
10. Known sensitivity to Busulfan.
11. Confirmation of an infectious disease by deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) positive at time of assessment for the following:
* HIV-1,
* Hepatitis B,
* Parvovirus B19.
12. The subject is pregnant or has a major congenital anomaly.
13. Is likely to require treatment during the study with drugs that are not permitted by the study protocol.
14. The subject has previously received another form of gene therapy.

Contacts and Locations

Study Contact

Satiro De Oliveira, MD

CONTACT

1-310-825-6708

sdeoliveira@mednet.ucla.edu

Augustine Fernandes, PhD

CONTACT

1-310-267-4948

afernandes@mednet.ucla.edu

Principal Investigator

Satiro De Oliveira, MD

PRINCIPAL_INVESTIGATOR

Assistant Professor

Study Locations (Sites)

University of California, Los Angeles (UCLA)

Los Angeles, California, 90095

United States

Collaborators and Investigators

Sponsor: University of California, Los Angeles

Satiro De Oliveira, MD, PRINCIPAL_INVESTIGATOR, Assistant Professor

Study Record Dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Registration Dates

Study Start Date2023-01-04

Study Completion Date2026-12-31

Study Record Updates

Study Start Date2023-01-04

Study Completion Date2026-12-31

Terms related to this study

Keywords Provided by Researchers

Gene Therapy
Hematopoietic Stem Cell
Lentiviral Vector
Reduced Intensity Conditioning with Busulfan

Additional Relevant MeSH Terms

Adenosine Deaminase Severe Combined Immune Deficiency