RECRUITING

CAR-T Cell Therapy for Desensitization in Kidney Transplantation

Conditions

Kidney Transplant Kidney Failure End Stage Renal Failure on Dialysis CART-BCMA huCART-19 Highly sensitized cPRA UNOS waiting list End stage renal failure patients with cPRA >99.5%

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Study Overview

This clinical trial focuses on testing the efficacy of different digital interventions to promote re-engagement in cancer-related long-term follow-up care for adolescent and young adult (AYA) survivors of childhood cancer.

Description

This research study is for people who have been waiting for a kidney transplant for at least one year, and who have a cPRA of 99.5% or higher. Having a cPRA of 99.5% or higher means that your immune system would reject 99.5% of kidneys available for transplant. The study will test whether new products called Chimeric Antigen Receptor T Cells (CAR T Cells), when given with chemotherapy, is safe and will reduce cPRA. The main study will last up to 2 years: Participants will have up to 30 clinic or hospital visits over a one-year period. If a transplant takes place, there will be 9 more visits after transplant. Long term follow up is required by the Food and Drug Administration (FDA) for 15 years after receiving CAR T cell. The primary objective is to evaluate the safety and feasibility of administering CART BCMA + huCART-19 following lymphodepletion, including determination of optimal tolerated regimen (OTR) and/or recommended phase 2 regimen, according to the incidence of dose limiting toxicity (DLT) in highly sensitized patients awaiting kidney transplant.

Official Title

Autologous Chimeric Antigen Receptor Engineered T Cell Immunotherapy for Desensitization in Patients Awaiting Kidney Transplantation

Quick Facts

Study Start:2024-05-09

Study Completion:2042-12-15

Study Type:Not specified

Phase:Not Applicable

Enrollment:Not specified

Status:RECRUITING

Study ID

NCT06056102

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Ages Eligible for Study:18 Years to 65 Years

Sexes Eligible for Study:ALL

Accepts Healthy Volunteers:No

Standard Ages:ADULT, OLDER_ADULT

Inclusion Criteria	Exclusion Criteria
1. Male or female patients aged 18-65 years with kidney failure requiring hemodialysis. 2. UNOS listed for kidney transplant for at least 1 year. 3. Patients must meet one of the following two criteria: 1. Protocol-specific cPRA ≥99.5% AND no suitable living donor, ineligible for kidney paired donation programs, have blood group Type O or B, and predictive of a positive virtual crossmatch to an available deceased donor 2. Protocol-specific cPRA ≥99.9% Protocol-specific cPRA must be rounded from three significant figures measured ≤90 days from the time of enrollment (i.e., cPRA of 0.994500 or 0.998500 would be eligible) using the web-based OPTN cPRA calculator (https://optn.transplant.hrsa.gov/resources/allocation-calculators/cpra-calculator/); accounting for HLA-A, -B, -C, -DRB1, -DRB3/4/5, and -DQB1 Luminex Single Antigen Beads (SAB) with MFI ≥3000; 1 archived sample within 6 months of screening required. 4. Based on center-specific listing policies, a cPRA in UNet Waitlist that is ≥99.5% (the candidate must be eligible for additional priority of kidneys equivalent to individuals with a 100% cPRA) 5. Able to understand and give written informed consent to participate in all aspects of the study. 6. Willing to stay within 2 hours of the home study site for at least 28 days after the last T cell infusion 7. Subjects of reproductive potential must agree to use contraception (see Section 5.6) for at least one year after CAR T Cell infusion 8. In the absence of contraindication, vaccinations must be up to date per the DAIT Guidance for Patients in Transplant Trials and include TdAP 9. Positive for EBV capsid IgG 10. Negative testing for latent TB infection within 3 months prior to enrollment. Testing should be conducted using either a PPD or interferon-gamma release assay (i.e. QuantiFERON-TB, T-SPOT.TB). Patients with a positive test for latent TB infection must complete appropriate therapy for LTBI. A subject is considered eligible only if they have a negative test for LTBI within 3 months prior to enrollment OR they have appropriately completed LTBI therapy prior to transplant. Latent TB infection treatment regimens should be among those endorsed by the CDC 11. Hemoglobin \>9g/dL 12. ANC ≥ 1,800/μL 13. Absolute Lymphocyte Counts 1000/μL or CD3 T cell Count 900 /μL 14. Platelet count ≥150,000/μL	1. Subjects with indwelling catheters as primary access for hemodialysis 2. Previous solid organ (except kidney) or bone marrow transplant 3. BMI \>30 kg/m2 4. Subjects who have preserved or oliguric urine output \> 100 cc/day with history of recurrent UTI (2 in 6 months or 3 in 1 year, see study definitions) 5. Subjects described in exclusion #4 with structural disease such as polycystic kidney disease, obstructive uropathy with nephrolithiasis or those otherwise at higher risk of urinary tract infections. Anuric subjects with structural kidney disease are not excluded 6. Known active current or history of invasive fungal infection, or non-tuberculous mycobacterial infection. Any infection requiring hospitalization and IV antibiotics within 4 weeks of screening or PO antibiotics within 2 weeks 7. History of HIV, chronic HBV, or chronic HCV, regardless of treatment 8. Negative CMV serology 9. Detectible viral load HBV, HCV, CMV, EBV, BK or SARS-CoV2 by PCR 10. Any B cell depleting or monoclonal antibody therapy within 6 months prior to enrollment 11. Receiving ongoing immunosuppression including corticosteroids, intravenous immunoglobulin, cyclophosphamide, tacrolimus, mycophenolic acid, or azathioprine from 90 days prior to study entry 12. Active auto-immune disease, including connective tissue disease, uveitis, sarcoidosis, inflammatory bowel disease, or multiple sclerosis, or have a history of severe (as judged by the investigator) autoimmune disease requiring prolonged immunosuppressive therapy, except for renal-limited autoimmune conditions without risk for systemic manifestations (e.g. IgA nephropathy) 13. Any chronic illness requiring uninterrupted anti-coagulation or anti-platelet therapy 14. History of cirrhosis or severe liver disease, including abnormal liver profile (aspartate aminotransferase \[AST\], alanine aminotransferases \[ALT\] or total bilirubin \> 3 times upper limit of normal at screening (except for patients in whom hyperbilirubinemia is attributed to Gilbert's syndrome) 15. History of sickle cell disease, or systemic amyloidosis 16. Cardiac clearance for transplant \> 6 months old and/or any of the following: NYHA Class III or IV heart failure, unstable angina, left ventricular ejection fraction \< 40%, a history of recent (within 6 months) myocardial infarction or implantable cardioverter/ defibrillators and/or biventricular pacing. 17. Moderate-severe pulmonary function abnormality, defined as resting oxygen saturation \<92% on room air or FEV1, TLC, or DLCO (after correction for hemoglobin) \<50% of predicted values 18. Patients who have received any live vaccine within 30 days of planned leukapheresis 19. Treatment with any investigational agent within 4 weeks (or 5 half-lives of investigational drug, whichever is longer) of screening 20. Pregnant, currently breastfeeding, or planning to become pregnant during the primary or post-transplant follow up of the study. 21. Past or current social or medical problems; or findings from physical examination or laboratory testing that are not listed above, which in the opinion of investigator, may pose additional risks from participation in the study, may interfere with the participant's ability to comply with study requirements or that may impact the quality or interpretation of the data obtained from the study 1. If the subject is positive for influenza, Tamiflu® or equivalent should be administered per package insert. The subject must complete treatment and symptoms must be improving and either resolved or nearly resolved in the judgment of the treating investigator prior to receiving lymphodepleting chemotherapy and CAR T cells. Repeat influenza testing is not required prior to initiating lymphodepleting chemotherapy and CAR T cell infusion. 2. If the subject tests positive for SARS-CoV-2, the subject will be managed per institutional practice. Subject will be eligible to initiate lymphodepleting chemotherapy and CAR T cell infusion once cleared from requirement for isolation according to institutional and/or CDC guidance. 3. If testing is positive for another respiratory virus (e.g., as part of a multiplex respiratory pathogen panel in the course of testing for influenza or SARS-CoV-2), the lymphodepleting chemotherapy and CAR T cell infusion will be delayed for at least 7 days to be sure clinical symptoms of a viral infection do not develop. If clinical symptoms develop, the lymphodepleting chemotherapy and CAR T cell infusion will be delayed until resolution of these symptoms. 1. Subjects must not have developed deterioration in performance status or overall clinical condition or new laboratory abnormalities that would, in the opinion of the treating investigator, render it unsafe to proceed with CAR T cell infusion. The following are specific conditions that warrant delaying CAR T cell infusion: 1. Requirement for supplemental oxygen to maintain peripheral oxygen saturation ≥95%. 2. Presence of clinically significant radiographic abnormalities on chest x-ray. Chest x-ray is not required to evaluate for radiographic abnormalities in the absence of suggestive symptoms or exam findings. 3. New cardiac arrhythmia not controlled with medical management. EKG is not required to evaluate for arrhythmia in the absence of suggestive symptoms or exam findings. 4. Hypotension requiring vasopressor support. 5. Active infection: Diagnostic test results indicating new bacterial, fungal, or viral infection within prior 48 hours. 2. Subjects must have adhered to restrictions on pre-infusion therapy.

Inclusion Criteria

Exclusion Criteria

1. Male or female patients aged 18-65 years with kidney failure requiring hemodialysis.
2. UNOS listed for kidney transplant for at least 1 year.
3. Patients must meet one of the following two criteria:
1. Protocol-specific cPRA ≥99.5% AND no suitable living donor, ineligible for kidney paired donation programs, have blood group Type O or B, and predictive of a positive virtual crossmatch to an available deceased donor
2. Protocol-specific cPRA ≥99.9% Protocol-specific cPRA must be rounded from three significant figures measured ≤90 days from the time of enrollment (i.e., cPRA of 0.994500 or 0.998500 would be eligible) using the web-based OPTN cPRA calculator (https://optn.transplant.hrsa.gov/resources/allocation-calculators/cpra-calculator/); accounting for HLA-A, -B, -C, -DRB1, -DRB3/4/5, and -DQB1 Luminex Single Antigen Beads (SAB) with MFI ≥3000; 1 archived sample within 6 months of screening required.
4. Based on center-specific listing policies, a cPRA in UNet Waitlist that is ≥99.5% (the candidate must be eligible for additional priority of kidneys equivalent to individuals with a 100% cPRA)
5. Able to understand and give written informed consent to participate in all aspects of the study.
6. Willing to stay within 2 hours of the home study site for at least 28 days after the last T cell infusion
7. Subjects of reproductive potential must agree to use contraception (see Section 5.6) for at least one year after CAR T Cell infusion
8. In the absence of contraindication, vaccinations must be up to date per the DAIT Guidance for Patients in Transplant Trials and include TdAP
9. Positive for EBV capsid IgG
10. Negative testing for latent TB infection within 3 months prior to enrollment. Testing should be conducted using either a PPD or interferon-gamma release assay (i.e. QuantiFERON-TB, T-SPOT.TB). Patients with a positive test for latent TB infection must complete appropriate therapy for LTBI. A subject is considered eligible only if they have a negative test for LTBI within 3 months prior to enrollment OR they have appropriately completed LTBI therapy prior to transplant. Latent TB infection treatment regimens should be among those endorsed by the CDC
11. Hemoglobin \>9g/dL
12. ANC ≥ 1,800/μL
13. Absolute Lymphocyte Counts 1000/μL or CD3 T cell Count 900 /μL
14. Platelet count ≥150,000/μL

1. Subjects with indwelling catheters as primary access for hemodialysis
2. Previous solid organ (except kidney) or bone marrow transplant
3. BMI \>30 kg/m2
4. Subjects who have preserved or oliguric urine output \> 100 cc/day with history of recurrent UTI (2 in 6 months or 3 in 1 year, see study definitions)
5. Subjects described in exclusion #4 with structural disease such as polycystic kidney disease, obstructive uropathy with nephrolithiasis or those otherwise at higher risk of urinary tract infections. Anuric subjects with structural kidney disease are not excluded
6. Known active current or history of invasive fungal infection, or non-tuberculous mycobacterial infection. Any infection requiring hospitalization and IV antibiotics within 4 weeks of screening or PO antibiotics within 2 weeks
7. History of HIV, chronic HBV, or chronic HCV, regardless of treatment
8. Negative CMV serology
9. Detectible viral load HBV, HCV, CMV, EBV, BK or SARS-CoV2 by PCR
10. Any B cell depleting or monoclonal antibody therapy within 6 months prior to enrollment
11. Receiving ongoing immunosuppression including corticosteroids, intravenous immunoglobulin, cyclophosphamide, tacrolimus, mycophenolic acid, or azathioprine from 90 days prior to study entry
12. Active auto-immune disease, including connective tissue disease, uveitis, sarcoidosis, inflammatory bowel disease, or multiple sclerosis, or have a history of severe (as judged by the investigator) autoimmune disease requiring prolonged immunosuppressive therapy, except for renal-limited autoimmune conditions without risk for systemic manifestations (e.g. IgA nephropathy)
13. Any chronic illness requiring uninterrupted anti-coagulation or anti-platelet therapy
14. History of cirrhosis or severe liver disease, including abnormal liver profile (aspartate aminotransferase \[AST\], alanine aminotransferases \[ALT\] or total bilirubin \> 3 times upper limit of normal at screening (except for patients in whom hyperbilirubinemia is attributed to Gilbert's syndrome)
15. History of sickle cell disease, or systemic amyloidosis
16. Cardiac clearance for transplant \> 6 months old and/or any of the following: NYHA Class III or IV heart failure, unstable angina, left ventricular ejection fraction \< 40%, a history of recent (within 6 months) myocardial infarction or implantable cardioverter/ defibrillators and/or biventricular pacing.
17. Moderate-severe pulmonary function abnormality, defined as resting oxygen saturation \<92% on room air or FEV1, TLC, or DLCO (after correction for hemoglobin) \<50% of predicted values
18. Patients who have received any live vaccine within 30 days of planned leukapheresis
19. Treatment with any investigational agent within 4 weeks (or 5 half-lives of investigational drug, whichever is longer) of screening
20. Pregnant, currently breastfeeding, or planning to become pregnant during the primary or post-transplant follow up of the study.
21. Past or current social or medical problems; or findings from physical examination or laboratory testing that are not listed above, which in the opinion of investigator, may pose additional risks from participation in the study, may interfere with the participant's ability to comply with study requirements or that may impact the quality or interpretation of the data obtained from the study
1. If the subject is positive for influenza, Tamiflu® or equivalent should be administered per package insert. The subject must complete treatment and symptoms must be improving and either resolved or nearly resolved in the judgment of the treating investigator prior to receiving lymphodepleting chemotherapy and CAR T cells. Repeat influenza testing is not required prior to initiating lymphodepleting chemotherapy and CAR T cell infusion.
2. If the subject tests positive for SARS-CoV-2, the subject will be managed per institutional practice. Subject will be eligible to initiate lymphodepleting chemotherapy and CAR T cell infusion once cleared from requirement for isolation according to institutional and/or CDC guidance.
3. If testing is positive for another respiratory virus (e.g., as part of a multiplex respiratory pathogen panel in the course of testing for influenza or SARS-CoV-2), the lymphodepleting chemotherapy and CAR T cell infusion will be delayed for at least 7 days to be sure clinical symptoms of a viral infection do not develop. If clinical symptoms develop, the lymphodepleting chemotherapy and CAR T cell infusion will be delayed until resolution of these symptoms.
1. Subjects must not have developed deterioration in performance status or overall clinical condition or new laboratory abnormalities that would, in the opinion of the treating investigator, render it unsafe to proceed with CAR T cell infusion. The following are specific conditions that warrant delaying CAR T cell infusion:
1. Requirement for supplemental oxygen to maintain peripheral oxygen saturation ≥95%.
2. Presence of clinically significant radiographic abnormalities on chest x-ray. Chest x-ray is not required to evaluate for radiographic abnormalities in the absence of suggestive symptoms or exam findings.
3. New cardiac arrhythmia not controlled with medical management. EKG is not required to evaluate for arrhythmia in the absence of suggestive symptoms or exam findings.
4. Hypotension requiring vasopressor support.
5. Active infection: Diagnostic test results indicating new bacterial, fungal, or viral infection within prior 48 hours.
2. Subjects must have adhered to restrictions on pre-infusion therapy.

Contacts and Locations

Study Contact

Mary Kaminski, PA

CONTACT

215-349-8334

mary.kaminski@pennmedicine.upenn.edu

Principal Investigator

Vijay Bhoj, M.D., Ph.D.

STUDY_CHAIR

University of Pennsylvania Medical Center: Transplantation

Ali Naji, MD, Ph.D.

PRINCIPAL_INVESTIGATOR

University of Pennsylvania Medical Center: Transplantation

Alfred Garfall, MD

STUDY_CHAIR

University of Pennsylvania Medical Center: Transplantation

Study Locations (Sites)

Massachusetts General Hospital: Transplantation (Site #: 71107)

Boston, Massachusetts, 02114

United States

NYU Langone Health (Site #: 71177)

New York, New York, 10016

United States

University of Pennsylvania Medical Center (Site #: 71111)

Philadelphia, Pennsylvania, 19104

United States

Collaborators and Investigators

Sponsor: National Institute of Allergy and Infectious Diseases (NIAID)

Vijay Bhoj, M.D., Ph.D., STUDY_CHAIR, University of Pennsylvania Medical Center: Transplantation
Ali Naji, MD, Ph.D., PRINCIPAL_INVESTIGATOR, University of Pennsylvania Medical Center: Transplantation
Alfred Garfall, MD, STUDY_CHAIR, University of Pennsylvania Medical Center: Transplantation

Study Record Dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Registration Dates

Study Start Date2024-05-09

Study Completion Date2042-12-15

Study Record Updates

Study Start Date2024-05-09

Study Completion Date2042-12-15

Terms related to this study

Keywords Provided by Researchers

Kidney transplant
CART-BCMA
huCART-19
Highly sensitized
cPRA
UNOS waiting list
End stage renal failure patients with cPRA >99.5%

Additional Relevant MeSH Terms

Kidney Transplant
Kidney Failure
End Stage Renal Failure on Dialysis