Efficacy of Recombinant Bovine Lactoferrin (rbLf) in Iron Regulation

Description

Overall Objective: To determine the efficacy of rbLf supplementation in a healthy adult population, specifically with regard to iron regulation (primary), gut health (secondary), and immune function (secondary). Purpose: The purpose of this study is to determine the effects of rbLf on iron regulation, exercise performance, gut health, and immune function as compared to cow's milk derived bLf. Participants: To account for an approximate \~10% dropout rate (and rounding up to ensure equal number of enrolled participants per group), n=25 per group for males (N=50) and n=30 per group for females (N=60) will be enrolled for a total sample size of N=110. Procedures: Participation in this study will include 6 in-person visits over 14 weeks. Anemia will be checked and excluded at the first visit using a single finger prick, followed by a 10-20 min exercise test on a treadmill to evaluate exercise capacity. On visit 2 participants will perform three 3-15 minute exercise tests on a treadmill with rest in between each test in order to determine exercise performance. A finger prick will be performed before and after each run to determine lactate levels. After visits 2 participants will be randomized to their respective supplement group recombinant Bovine Lactoferrin supplement (rbLf) (Male and Fame), a bovine-milk derived Lactoferrin supplement (Female), or a placebo (Male) once daily for 4 weeks. Blood samples will be obtained prior to and after each supplement phase to determine change in iron levels and red blood cell capacity. After a 2-week washout participants will return to complete the same testing outcomes and will receive the subsequent supplement. At visit 6 a final blood sample will be collected.

Conditions

Exercise Performance

Study Overview

Study Details

Study overview

Overall Objective: To determine the efficacy of rbLf supplementation in a healthy adult population, specifically with regard to iron regulation (primary), gut health (secondary), and immune function (secondary). Purpose: The purpose of this study is to determine the effects of rbLf on iron regulation, exercise performance, gut health, and immune function as compared to cow's milk derived bLf. Participants: To account for an approximate \~10% dropout rate (and rounding up to ensure equal number of enrolled participants per group), n=25 per group for males (N=50) and n=30 per group for females (N=60) will be enrolled for a total sample size of N=110. Procedures: Participation in this study will include 6 in-person visits over 14 weeks. Anemia will be checked and excluded at the first visit using a single finger prick, followed by a 10-20 min exercise test on a treadmill to evaluate exercise capacity. On visit 2 participants will perform three 3-15 minute exercise tests on a treadmill with rest in between each test in order to determine exercise performance. A finger prick will be performed before and after each run to determine lactate levels. After visits 2 participants will be randomized to their respective supplement group recombinant Bovine Lactoferrin supplement (rbLf) (Male and Fame), a bovine-milk derived Lactoferrin supplement (Female), or a placebo (Male) once daily for 4 weeks. Blood samples will be obtained prior to and after each supplement phase to determine change in iron levels and red blood cell capacity. After a 2-week washout participants will return to complete the same testing outcomes and will receive the subsequent supplement. At visit 6 a final blood sample will be collected.

Efficacy of Recombinant Bovine Lactoferrin (rbLf) in Iron Regulation

Efficacy of Recombinant Bovine Lactoferrin (rbLf) in Iron Regulation

Condition
Exercise Performance
Intervention / Treatment

-

Contacts and Locations

Chapel Hill

Applied Physiology Laboratory, Chapel Hill, North Carolina, United States, 27599

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

For general information about clinical research, read Learn About Studies.

Eligibility Criteria

  • * 18-42 years
  • * Body mass index less than 35 kg/m\^2
  • * Finger prick hemoglobin levels fitting within "normal range"
  • * Males: 14-18g/dL
  • * Females: 12-16g/dL
  • * Active: meeting American College of Sports Medicine exercise guidelines or exercising more than 2 days per week of
  • * Pre-menopausal: experiencing a regular period with no signs of perimenopause or menopause.
  • * Clinically diagnosed iron-deficiency anemia, pagophagia, hemochromatosis, or other blood or iron related medical conditions.
  • * Allergy or intolerance to milk, egg, soy, peanut, wheat, coconut, or almond dairy
  • * Smokers or vapers of nicotine or nicotine products
  • * Immunocompromised or diagnosed with Type I or II diabetes
  • * Irritable Bowel Disease, Crohn's disease, Celiacs
  • * Bowel movements less than three times per week, or clinically constipated
  • * Oral contraceptive users that have combined iron supplementation, or non-monophasic oral contraceptive users.
  • * Pregnant or nursing
  • * Chronic eczema or clinically diagnosed asthma.
  • * Current antibiotic use or antibiotic use within the past 6 weeks
  • * If flu, corona virus, or cold occurs, subject will remain in the study with their original scheduled visits, but immune values will be excluded.
  • * Vegan (due to supplement ingredients).

Ages Eligible for Study

18 Years to 42 Years

Sexes Eligible for Study

ALL

Accepts Healthy Volunteers

Yes

Collaborators and Investigators

University of North Carolina, Chapel Hill,

Abbie Smith-Ryan, PhD, PRINCIPAL_INVESTIGATOR, University of North Carolina, Chapel Hill

Study Record Dates

2025-11