Effects of Beverage Volume, Glycerol Concentration, and Sodium Concentration on Fluid Balance in Healthy Euhydrated Men and Women at Rest

Eligibility Criteria

• * Subject is male or female
• * If female, subject is not pregnant
• * Subject is 18-50 years of age, inclusive
• * Subject is at least recreationally active (engaged in light to moderate-intensity, intermittent, or steady-state exercise at least 3 days per week for at least 30 min at a time)
• * Subject does not smoke (or has quit for at least 6 months)
• * Subject is not taking medication that may interfere with the study (e.g., diuretics)
• * Subject has no health conditions that would interfere with the study (e.g. cardiovascular, renal, or metabolic diseases)
• * Subject is willing to avoid alcohol consumption 24 hours prior to visit(s)
• * Subject is willing to fast overnight (\~8-12 hours)
• * Subject is willing to refrain from vigorous exercise for 24 hours
• * Subject is willing to eat the exact same food the day prior to each visit to the laboratory
• * Provision of written consent to participate
• * Subject has participated in a clinical trial within the past 30 days
• * Subject has participated in any PepsiCo trial within past 6 months
• * Subject has a condition or is taking medication that the investigator believes would interfere with his/her ability to provide informed consent, comply with the study protocol, which might confound the interpretation of the study results, or put the person at undue risk
• * Subject is employed by, or has a parent, guardian, or other immediate family member employed by a company that manufactures any products that compete with any Gatorade product. If subject is unsure if a company would be considered a competitor to Gatorade, they will be asked to please let the study investigator know the name of the other company and the nature of their relationship to that company before they sign the informed consent.