ACTIVE_NOT_RECRUITING

Myeloablative Conditioning Orca-T & Allogeneic Donor-Derived CD19/CD22-CAR TCells in B-Cell ALL

Conditions

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Study Overview

This clinical trial focuses on testing the efficacy of different digital interventions to promote re-engagement in cancer-related long-term follow-up care for adolescent and young adult (AYA) survivors of childhood cancer.

Description

To assess the safety of administering allogenic, donor-derived CD19/CD22-CAR T cells that meet established release specifications in adults with B-cell ALL following a myeloablative conditioning regimen and Orca-T to determine if this will augment graft versus leukemia without increasing acute GVHD or graft failure.

Official Title

Phase I Trial Evaluating the Safety of Myeloablative Conditioning, Orca-T, and Allogeneic, Donor-Derived CD19/CD22-CAR (Chimeric Antigen Receptor) T Cells in Adults With B-Cell Acute Lymphoblastic Leukemia (ALL)

Quick Facts

Study Start:2022-08-18

Study Completion:2026-05

Study Type:Not specified

Phase:Not Applicable

Enrollment:Not specified

Status:ACTIVE_NOT_RECRUITING

Study ID

NCT05507827

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Ages Eligible for Study:18 Years to 65 Years

Sexes Eligible for Study:ALL

Accepts Healthy Volunteers:No

Standard Ages:ADULT, OLDER_ADULT

Inclusion Criteria	Exclusion Criteria
* Subjects in CR must have a history of chemotherapy refractory disease defined as progression or stable disease after one line of chemotherapy, or relapsed disease after achieving prior CR OR must have other high risk ALL features including: CRLF2 rearrangement, Ph-like phenotype, MLL/KMT2a rearrangement, or hypodiploid karyotype. * Subjects with persistent or relapsed minimal residual disease (MRD) (by flow cytometry, PCR, FISH, or next generation sequencing) require verification of MRD in the peripheral blood or bone marrow on two occasions at least 2 weeks apart. * Subjects with active ALL (defined as \>=5% bone marrow blasts, circulating blasts, or extramedullary disease) are eligible. * Age ≥ 18 and ≤ 65 years (i.e., from age 18 to \< 66 years old) at the time of enrollment * Eastern cooperative oncology group (ECOG) performance status of 0, 1, or 2; or Karnofsky ≥ 60% * CD19 expression is required any time since diagnosis. CD19 expression may be detected by immunohistochemistry or by flow cytometry. The choice of whether to use flow cytometry or immunohistochemistry will be determined by what is the most easily available tissue sample in each subject. In general, immunohistochemistry will be used for lymph node biopsies, flow cytometry will be used for peripheral blood and bone marrow samples. Patients receiving prior CD19 CAR T cell or blinatumomab are eligible if there is no documented history of CD19 negativity on the malignant cells. * Subjects must have an HLA matched related donor willing to undergo unstimulated apheresis for T cell collection for CAR T cell generation followed by GCSF mobilized apheresis for HSC/Treg graft. * Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typed using DNA-based high-resolution methods * Subjects must have adequate organ function measured by: * Cardiac: Cardiac ejection fraction at rest ≥ 45% * Hepatic: * Total bilirubin \< 2 times upper limit of normal (ULN) (patients with Gilbert's syndrome may be included where hemolysis has been excluded and with approval of the study PI) * ALT/AST \<= 3 times ULN * Renal: Calculated creatinine clearance ≥ 50 mL/min or serum creatinine \< 2.0 mg/dL * Pulmonary: Diffusing capacity of the lung for carbon monoxide (DLCO) (adjusted for hemoglobin) ≥ 50% * CNS: Subjects with CNS involvement are eligible as long as there are no overt signs or symptoms that in the evaluation of the investigator would mask or interfere with the neurological assessment of toxicity. * Negative serum or urine beta-HCG test in females of childbearing potential within 3 weeks of registration * Subjects of childbearing or child fathering potential must be willing to practice birth control from the time of enrollment on this study and for twelve (12) months after receiving the preparative conditioning regimen. * Must be able to give informed consent. Legal authorized representative (LAR) is permitted if subject is cognitively able to provide verbal assent. * Age ≥ 18 and ≤ 75 years at time of enrollment * Karnofsky performance status of ≥ 70% defined by institutional standards * Willing to donate for two separate apheresis collections (T cells and PBSC) * Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typed using DNA-based high-resolution methods * Negative serum or urine beta-HCG test in females of childbearing potential within 2 weeks of first apheresis * Seronegative for HIV-1 RNA PCR; HIV 1 and HIV 2 Ab (antibody); HTLV-1 and HTLV-2 Ab; PCR negative or sAg (surface antigen) negative for hepatitis B; negative for the Treponema pallidum antibody Syphilis screen; and negative for HIV-1 and hepatitis C by nucleic acid testing (NAT) within 40 days of donor apheresis procedures. * In the case that T pallidum antibody tests are positive, donors must: * Be evaluated and show no evidence of syphilis infection of any stage by physical exam and history * Have completed effective antibiotic therapy to treat syphilis * Have a documented negative non-treponemal test (such as RPR) or in the case of a positive non-treponemal test must be evaluated by an infectious disease expert to evaluate for alternative causes of test positivity and confirm no evidence of active syphilitic disease	* History of other malignancy unless disease free for at least 3 years. At the discretion of the Principal Investigator, subjects in remission for 1-2 years prior to enrollment may be deemed eligible after considering the nature of other malignancy, likelihood of recurrence for one year following therapy, and impact of prior treatment on risk of CD19/CD22-CAR T cells and Treg graft. Subjects in remission \<1 year are not eligible. The following exceptions apply: * Nonmelanoma skin cancer or carcinoma in situ (e.g. cervix, bladder, breast) is eligible. * Hormonal therapy in subjects in remission \>1 year will be allowed. * Patients who have undergone a prior allogeneic or autologous stem cell transplant. * Recipient positive anti-donor HLA antibodies against a mismatched allele in the selected donor determined by either: * a positive crossmatch test of any titer (by complement-dependent cytotoxicity or flow cytometric testing), or * the presence of anti-donor HLA antibody to any of the following HLA loci: HLA-A, -B, -C, -DRB1, -DQB1, -DQA1, -DPB1, or -DPA1, with mean fluorescence intensity (MFI) \>1000 by solid phase immunoassay * Presence of fungal, bacterial, viral, or other infection that is uncontrolled. Simple UTI and uncomplicated bacterial pharyngitis are permitted if responding to active treatment. * Known history of infection with any of the following: * HIV * Hepatitis B (HBsAg positive) \\ * Hepatitis C virus (anti HCV positive) \\ * Currently receiving corticosteroids or other immunosuppressive therapy. Topical corticosteroids or oral systemic corticosteroid doses less than or equal to 10 mg/day are allowed. * Planned pharmaceutical in vivo or ex vivo T cell depletion, e.g., post-transplant cyclophosphamide (Cy), peri-transplant anti-thymocyte globulin (ATG), or alemtuzumab. For patients that have previously been exposed to a T cell-depleting agent, a 5-half-life washout of the agent must occur prior to planned Transplant Day 0. * Hyperleukocytosis (≥ 50,000 blasts/μL) or rapidly progressive disease that in the estimation of the investigator and sponsor would compromise ability to complete study therapy. * History of myocardial infarction, cardiac angioplasty or stenting, unstable angina, or other clinically significant cardiac disease within 12 months of enrollment. * Pregnant or breast feeding * Patients with known autoimmune disease requiring the use of systemic immunosuppressive therapy within the last year * Presence of a seizure disorder, cerebrovascular ischemia/hemorrhage, dementia, cerebellar disease, or any autoimmune disease with CNS involvement that in the judgment of the investigator may impair the ability to evaluate neurotoxicity. * Any medical condition that in the judgement of the investigator is likely to interfere with assessment of safety or efficacy of study treatment * Evidence of active infection * Seropositive for HIV-1 or -2, HTLV-1 or -2 * Positive PCR test results indicating acute or chronic HBV infection. Patients with isolated HBV core antibody positivity will not be excluded. Donors whose HBV infection status cannot be determined conclusively by serologic test results (www.cdc.gov/hepatitis/hbv/pdfs/serologicchartv8.pdf) must be negative for HBV by PCR to be eligible for study participation. * Potential for Zika virus infection as defined as any of the following: * Medical diagnosis of Zika virus infection in the past 6 months * Residence in, or travel to, an area with active Zika virus transmission within the past 6 months. * Unprotected sex within the past 6 months with a person who is known to have either of the risk factors listed above (donor exclusion criterion 5.a or 5.b) * Donors determined to be ineligible based on the results of Zika virus screening may be determined to be eligible if: * Pregnant or breastfeeding female * Medical, physical, or psychological reason that would place the donor at increased risk for complications from growth factor or leukapheresis.

Inclusion Criteria

Exclusion Criteria

* Subjects in CR must have a history of chemotherapy refractory disease defined as progression or stable disease after one line of chemotherapy, or relapsed disease after achieving prior CR OR must have other high risk ALL features including: CRLF2 rearrangement, Ph-like phenotype, MLL/KMT2a rearrangement, or hypodiploid karyotype.
* Subjects with persistent or relapsed minimal residual disease (MRD) (by flow cytometry, PCR, FISH, or next generation sequencing) require verification of MRD in the peripheral blood or bone marrow on two occasions at least 2 weeks apart.
* Subjects with active ALL (defined as \>=5% bone marrow blasts, circulating blasts, or extramedullary disease) are eligible.
* Age ≥ 18 and ≤ 65 years (i.e., from age 18 to \< 66 years old) at the time of enrollment
* Eastern cooperative oncology group (ECOG) performance status of 0, 1, or 2; or Karnofsky ≥ 60%
* CD19 expression is required any time since diagnosis. CD19 expression may be detected by immunohistochemistry or by flow cytometry. The choice of whether to use flow cytometry or immunohistochemistry will be determined by what is the most easily available tissue sample in each subject. In general, immunohistochemistry will be used for lymph node biopsies, flow cytometry will be used for peripheral blood and bone marrow samples. Patients receiving prior CD19 CAR T cell or blinatumomab are eligible if there is no documented history of CD19 negativity on the malignant cells.
* Subjects must have an HLA matched related donor willing to undergo unstimulated apheresis for T cell collection for CAR T cell generation followed by GCSF mobilized apheresis for HSC/Treg graft.
* Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typed using DNA-based high-resolution methods
* Subjects must have adequate organ function measured by:
* Cardiac: Cardiac ejection fraction at rest ≥ 45%
* Hepatic:
* Total bilirubin \< 2 times upper limit of normal (ULN) (patients with Gilbert's syndrome may be included where hemolysis has been excluded and with approval of the study PI)
* ALT/AST \<= 3 times ULN
* Renal: Calculated creatinine clearance ≥ 50 mL/min or serum creatinine \< 2.0 mg/dL
* Pulmonary: Diffusing capacity of the lung for carbon monoxide (DLCO) (adjusted for hemoglobin) ≥ 50%
* CNS: Subjects with CNS involvement are eligible as long as there are no overt signs or symptoms that in the evaluation of the investigator would mask or interfere with the neurological assessment of toxicity.
* Negative serum or urine beta-HCG test in females of childbearing potential within 3 weeks of registration
* Subjects of childbearing or child fathering potential must be willing to practice birth control from the time of enrollment on this study and for twelve (12) months after receiving the preparative conditioning regimen.
* Must be able to give informed consent. Legal authorized representative (LAR) is permitted if subject is cognitively able to provide verbal assent.
* Age ≥ 18 and ≤ 75 years at time of enrollment
* Karnofsky performance status of ≥ 70% defined by institutional standards
* Willing to donate for two separate apheresis collections (T cells and PBSC)
* Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typed using DNA-based high-resolution methods
* Negative serum or urine beta-HCG test in females of childbearing potential within 2 weeks of first apheresis
* Seronegative for HIV-1 RNA PCR; HIV 1 and HIV 2 Ab (antibody); HTLV-1 and HTLV-2 Ab; PCR negative or sAg (surface antigen) negative for hepatitis B; negative for the Treponema pallidum antibody Syphilis screen; and negative for HIV-1 and hepatitis C by nucleic acid testing (NAT) within 40 days of donor apheresis procedures.
* In the case that T pallidum antibody tests are positive, donors must:
* Be evaluated and show no evidence of syphilis infection of any stage by physical exam and history
* Have completed effective antibiotic therapy to treat syphilis
* Have a documented negative non-treponemal test (such as RPR) or in the case of a positive non-treponemal test must be evaluated by an infectious disease expert to evaluate for alternative causes of test positivity and confirm no evidence of active syphilitic disease

* History of other malignancy unless disease free for at least 3 years. At the discretion of the Principal Investigator, subjects in remission for 1-2 years prior to enrollment may be deemed eligible after considering the nature of other malignancy, likelihood of recurrence for one year following therapy, and impact of prior treatment on risk of CD19/CD22-CAR T cells and Treg graft. Subjects in remission \<1 year are not eligible. The following exceptions apply:
* Nonmelanoma skin cancer or carcinoma in situ (e.g. cervix, bladder, breast) is eligible.
* Hormonal therapy in subjects in remission \>1 year will be allowed.
* Patients who have undergone a prior allogeneic or autologous stem cell transplant.
* Recipient positive anti-donor HLA antibodies against a mismatched allele in the selected donor determined by either:
* a positive crossmatch test of any titer (by complement-dependent cytotoxicity or flow cytometric testing), or
* the presence of anti-donor HLA antibody to any of the following HLA loci: HLA-A, -B, -C, -DRB1, -DQB1, -DQA1, -DPB1, or -DPA1, with mean fluorescence intensity (MFI) \>1000 by solid phase immunoassay
* Presence of fungal, bacterial, viral, or other infection that is uncontrolled. Simple UTI and uncomplicated bacterial pharyngitis are permitted if responding to active treatment.
* Known history of infection with any of the following:
* HIV
* Hepatitis B (HBsAg positive) \*\*
* Hepatitis C virus (anti HCV positive) \*\*
* Currently receiving corticosteroids or other immunosuppressive therapy. Topical corticosteroids or oral systemic corticosteroid doses less than or equal to 10 mg/day are allowed.
* Planned pharmaceutical in vivo or ex vivo T cell depletion, e.g., post-transplant cyclophosphamide (Cy), peri-transplant anti-thymocyte globulin (ATG), or alemtuzumab. For patients that have previously been exposed to a T cell-depleting agent, a 5-half-life washout of the agent must occur prior to planned Transplant Day 0.
* Hyperleukocytosis (≥ 50,000 blasts/μL) or rapidly progressive disease that in the estimation of the investigator and sponsor would compromise ability to complete study therapy.
* History of myocardial infarction, cardiac angioplasty or stenting, unstable angina, or other clinically significant cardiac disease within 12 months of enrollment.
* Pregnant or breast feeding
* Patients with known autoimmune disease requiring the use of systemic immunosuppressive therapy within the last year
* Presence of a seizure disorder, cerebrovascular ischemia/hemorrhage, dementia, cerebellar disease, or any autoimmune disease with CNS involvement that in the judgment of the investigator may impair the ability to evaluate neurotoxicity.
* Any medical condition that in the judgement of the investigator is likely to interfere with assessment of safety or efficacy of study treatment
* Evidence of active infection
* Seropositive for HIV-1 or -2, HTLV-1 or -2
* Positive PCR test results indicating acute or chronic HBV infection. Patients with isolated HBV core antibody positivity will not be excluded. Donors whose HBV infection status cannot be determined conclusively by serologic test results (www.cdc.gov/hepatitis/hbv/pdfs/serologicchartv8.pdf) must be negative for HBV by PCR to be eligible for study participation.
* Potential for Zika virus infection as defined as any of the following:
* Medical diagnosis of Zika virus infection in the past 6 months
* Residence in, or travel to, an area with active Zika virus transmission within the past 6 months.
* Unprotected sex within the past 6 months with a person who is known to have either of the risk factors listed above (donor exclusion criterion 5.a or 5.b)
* Donors determined to be ineligible based on the results of Zika virus screening may be determined to be eligible if:
* Pregnant or breastfeeding female
* Medical, physical, or psychological reason that would place the donor at increased risk for complications from growth factor or leukapheresis.

Contacts and Locations

Principal Investigator

Lori Muffly, MD

PRINCIPAL_INVESTIGATOR

Stanford University

Study Locations (Sites)

Stanford Cancer Center

Palo Alto, California, 94305

United States

Collaborators and Investigators

Sponsor: Stanford University

Lori Muffly, MD, PRINCIPAL_INVESTIGATOR, Stanford University

Study Record Dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Registration Dates

Study Start Date2022-08-18

Study Completion Date2026-05

Study Record Updates

Study Start Date2022-08-18

Study Completion Date2026-05

Terms related to this study

Additional Relevant MeSH Terms

Lymphoid Leukemia